Composite

Part:BBa_K5461001:Design

Designed by: Jiang Nan   Group: iGEM24_LCG-China   (2024-09-25)


J23119-BBa_B0034- POI -Spycatcher- BBa_B1006


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SpyCatcher was chosen because it can form an isopeptide bond with SpyTag, which is much stronger than typical hydrogen bonds and hydrophobic interactions.


Source

spycatcher is a gene on the Streptococcus pyogenes genome. J23119, BBa_B0034, and BBa_B1006 are promoter, RBS, and terminator, respectively, contributed by iGEM.

References