Composite
Part:BBa_K5461001:Design
Designed by: Jiang Nan Group: iGEM24_LCG-China (2024-09-25)
J23119-BBa_B0034- POI -Spycatcher- BBa_B1006
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
SpyCatcher was chosen because it can form an isopeptide bond with SpyTag, which is much stronger than typical hydrogen bonds and hydrophobic interactions.
Source
spycatcher is a gene on the Streptococcus pyogenes genome. J23119, BBa_B0034, and BBa_B1006 are promoter, RBS, and terminator, respectively, contributed by iGEM.